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| Vigneron, Vassilia; Solliec, Gaelle; Montanie, Hélène; Renault, Tristan. |
| Since 1991, herpesvirus infections have been reported among larvae and juveniles of various bivalves. Most of the studies focused on detection of viral infections of economically important species. However, the persistence of bivalve herpesviruses; in the marine environment is poorly documented. The present study concerns the role of seawater parameters in Ostreid Herpesvirus I (OsHV-1) detection by polymerase chain reaction (PCR). Viral DNA extracted from purified particles or virions present in infected oyster larvae were detected by PCR after storage in different media at different temperatures. The lowest detection threshold was found using distilled water or Tris EDTA buffer. In seawater, the threshold was higher. The use of sterile media permitted... |
| Tipo: Text |
Palavras-chave: Temperature; PCR; Detection; Seawater; Viral DNA; Oyster herpesvirus 1. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-2909.pdf |
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| Pepin, Jean-francois; Solliec, Gaelle; Vigneron, Vassilia; Thebault, Anne; Lamothe, Julien; Renault, Tristan. |
| The presence of a herpes-type virus has been demonstrated in various species of marine shellfish (bivalves and gastropoda) throughout different regions of the world (Europe, North and South America, Asia, Australia), and is frequently associated with high mortality levels. Nevertheless, apart from the sanitary monitoring in mollusc farming, very little data is available about the detection of this type of virus in the natural populations of bivalves and about the persistence of such viruses in sea water. Various diagnostic tools have been developed in order to detect the oyster herpes virus (Ostreid herpesvirus, OsHV). The presence of this virus has been looked for by PCR in marine water samples coming from fattening ponds, in order to precise the natural... |
| Tipo: Text |
Palavras-chave: Levures; Eau de mer; Diagnostic; Huîtres; Mollusques; OsHV; Herpèsvirus. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-3716.pdf |
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